TLC habab fudud falanqaynta adag

TLC waa hoggaamiye ee arrimaha falanqaynta taranta iyo semi-taranta ee dawooyinka adag, dabiiciga ah, Biomedical, farsamada, kiimikada iyo walxaha kale oo badan. chromatography lakabka khafiif ah sidoo kale waa habka ugu heli karo falanqaynta mass of dhammaan fasalada walxaha.

Tani farsamada kala go'ida iyo falanqayn nidaamyada multi-qayb hubkaas ayaa loo isticmaalaa in laboratories koobnaanta aad u kala duwan iyo jihada: caafi-epidemioligal, xoolaha, agrochemical, cilmi baarista, foreensiga, iyo saldhigyada ilaalinta dhirta, Center for simayo iyo Bureau of Forensic Medicine.

Faa'iidooyinka ugu weyn ee chromatography dhuuban-lakabka, ku salaysan eluent mooshin (dareeraha analyte) ee lakabka sorbent ah iyada oo loogu yeero Ciidamada gaddoonka waa halleeyo lillaahinimada iyo naqo, geedi socodka chromatographic, shahaado sare oo sax ah falanqaynta iyo kharashka yar ee qalabka lagama maarmaanka ah.

TLC, isagoo shahaado sare ee dareenka (hoose marinka ogaanshaha) iyo selectivity, ogolaanaya si loo ogaado 10-20 walxaha ug ilaa 7%, taas oo ah mid aad u sarreeya. chromatography lakabka khafiif ah waxay noqon kartaa adsorption iyo qaybinta nooc. Waxaa ugu ballaaran loo isticmaalo waa ikhtiyaarka ugu horeysay.

Nuxurka habka been TLC guuro walax dareeraha sariirta sorbent iyo qaybinta aan ollogga ah ee qaybaha kala duwan sida ay sifooyinka iyo guryaha gaarka ah. Xaqiiqada ah in ay u taagoo of Halabuurka iyo qaab-dhismeedka adsorbed kala duwan (shubo) oo korka ka mid ah waslad khafiif ah oo adag chromatographic duwan. Sidaa darteed, nidaam multi-qayb adag waxaa si tartiib tartiib ah qaybaha kala shakhsiga.

Guuritaanka xawaare kala duwan oo ay la socdaan sariirta sorbent ah, qofka waxaa hore u soocay, qaybaha foomka chromatogram ah. kaalinta midab kasta oo u dhiganta kiimikada gaar ah. walax Biyuhu iftiinka ultraviolet sarkhaansan yihiin taas oo hannaanka ah xeryahooda bilaabaan inay fluoresce, ama la hoos geliyey reagent midabaynta daaweyn gaar ah.

Waxaa ka mid casri ah hababka gorfaynta GLC taagan. Habkani waxa lagu gartaa-soociddiisa iyo falanqaynta la isticmaalayo, sida wakiilka sorbing a dareere nonvolatile gaar ah, kuna waari dhexdeeda wax budada dhoobada waxaa ilmadeeda. Oo sidii dhexdhexaad ah wadista isticmaalay halkan kala duwan gaasas inert. Habkani waa kan ugu waxtarka badan ee waxbarasho ee isku dhiska adag ay ku jiraan boqolaal ka mid ah qaybaha kala duwan.

Kale oo casri ah oo hab baaritaan ka kooban walxaha qalafsan waa chromatography dareere ah. feature asaasiga ah ee habkan kooban yahay ee kala ah qaybaha shaqsi adigoo isticmaalaya kala duwan ee ay qaybinta u dhexeeya dhaqaajin karo (eluent) oo go'an weji (adsorbent). Habkani waxaa inta badan la isticmaalo ee falanqaynta xeryahooda kala duwan organic (lagu daro cuntada, sunta cayayaanka, sunta kala duwan, iwm). Sidoo kale, sida hab chromatographic waxaa loo isticmaalaa si ay u bartaan gacanta tayada dawooyinka, go'aan ka soo baxay steroids dheecaanka nafleyda, iyo falanqaynta kale amino acid.