Lala dayo immunosorbent: codsiga iyo muuqaalada

Lala dayo immunosorbent - baadhitaan shaybaadh, taas oo ku salaysan yahay aqoonsiga kaxeynayo "antigen antibody-" with a sumadaha enzymatic gaar ah in la aqoonsado, by beddelo midabka ah ee substrate ah. Ujeedada ugu weyn ee falanqaynta this - go'aanka joogitaanka mid ah waxyaabaha kala duwan ee reactions enzymatic ee shaybaarka baaritaanka.

Waayo, ogaanshaha cudur iyo antibodies immunoassay enzyme Nuurre Aw loo isticmaalo, tan iyo markii ay geedi socodka waa u fududahay in la kala saaro qaybaha of falcelinta iyo dib u dhigto qaar iyaga ka mid ah in wajiga adag.

Waxaa xusid mudan faa'iidooyinka soo socda waxbarasho shaybaarka this:

• dareen sare, taas oo lagu ogaan karaa xitaa tiro yar oo u taagoo of walxaha;

• hirgalin falanqaynta this isticmaali kartaa tirada ugu yar ee wax baaritaanka;

• Suurtagalnimada ee waqti dheer si ay u sii dhammaan qaybaha in loo baahan yahay si loo baadho,

• Easy si ay u qabtaan,

• helitaanka natiijooyinka xarkaha iyo muuqaal ah,

• dayo immunosorbent waa iswada heer kasta oo dareen-celinta;

• xirmooyinka kharash yar in loo isticmaalo cudurka.

faa'iidooyinka waxay ka dhigi isticmaalka ballaaran ee IFA meelaha kala duwan ee daawada, oo ay ku jiraan, waayo cudurka ee fayraska.

Habka ugu caamsan ee joojinta antibodies iyo cudur tahay ficil adsorption ah, markii ay u taagoo gaar ah ku xiran yihiin marxaladda adag (sabab u tahay isdhexgalka Madbacada qaranka ama hydrophobic ama by formation of bonds hydrogen).

Waa inaan sheegaa in immunoassay enzyme loo isticmaalo in laba xaaladood:

• in la ogaado cudur of wareer ah ee cudurada kala duwan;

• si loo ogaado jiritaanka unugyada in antigen ee sheyga bahal ah (waa in la ogaadaa in ELISA lagu ogaan karaa ku dhawaad cudurka kasta oo xubinta taranka).

saxan polystyrene Gaarka ah waxaa loo isticmaalay in lagu fuliyo serodiagnosis this. Waxay leedahay 96 godad on dhinacyada antigen adsorbed. Marka laga soo galo ee kiniin isku dayeen ku antibodies antigen gacanta ku xiran yihiin ku haboon. Intaa waxaa dheer ku xiga ee antibody a ku tilmaamay in ceelashii immunoglobulins aadanaha kordhiyay reaction gaar ah. In chromogen serum daaweynta ka dib (dheeh) kala duwan substrate midabaynta (iyadoo ay wada joogaan oo ka mid ah kaxeynayo dhiganta "antigen antibody-"). Waxaa xusid mudan in xoogga midabka waa si toos ah uu u dhigmaa qadarka antibodies.

ELISA Falanqaynta dhameysata qiyaaso cufnaanta indhaha ee dareeraha ah ee ceelasha. Sayidka isticmaalaan qalab gaar ah iyo astaamo u ekaansho ka caawiya tirin fiirsashada antibody ah. Waa in la xusuustaa in nidaamka baaritaanka kasta si ay u qabtaan falanqaynta this waxay leedahay qiimaha ay of tilmaamayaasha caadiga ah (this waxaa badanaa lagu qoray in natiijada, kuwaas oo loo gacan geliyey).

Isticmaalka ELISA ogaado waraabowga, hepatitis viral iyo HIV, iyo sidoo kale laga helay cytomegalovirus aadanaha jirka, Chlamydia, herpes (noocyada kala duwan). Falanqaynta Tani waxay kaloo ka caawisaa si loo ogaado heerka hoormoonka si loo ogaado calamado kansarka.

Halka laga baadho ee Moscow? Maanta, waxaa jira wax badan oo ka mid ah shaybaarada gaar ah, kuwaas oo bixiya tiro balaadhan oo ah fuliyo. Waayo, tilmaamaha fadlan la tasho dhakhtar oo ah xarunta caafimaadka ee deegaanka (gaar ah ama xirfadle, waxaad dveryaete). Iyada oo natiijada imtixaanka sidoo kale waa in uu takhtarka aado. Dhagayso hadalo uu on baahida loo qabo in wax daaweyn ah.