Kelli Bioscience:-waqtiga dhabta ah PCR

reaction silsilad polymerase Currently, Baadhista kelli hab loogu talagalay (PCR), taas oo ahayd wadadii uu horumarinta tiro badan oo ah hababka ogaanshaha cudurrada, oo ku salaysan go'aan ka soo baxay wax hidaha. Waayo, fulinta-waqtiga dhabta ah PCR isticmaalaya jab hiddo (gaar ahaan acid ee nucleic) ee wakiilka sababaya ah, oo waa inaan laga helin shakhsiyaad kale.

Mabda'a ah PCR waa isku dhufasho ee ah malaayiin jab DNA pathogene qaar ka mid ah jeer by DNA polymerase ah waax gaar ah, loo yaqaan cycler ee kaamerada, kaas oo bixiya noq-a xilliile taliskii heerkulka barnaamij siiyey.

Baadhista xanuunada PCR u ogolaanaya in ay soo celiyaan taxane ah oo amplifications, dayactirka hawl eulayska. Marka cod ku meel habsami leh, in mid ka mid ah wareegga tirada nuqulo ka mid ah xabbad gaar ah DNA labanlaabantay pathogene ah, ka dib markii soddon wareegyada - boqol iyo toban. Taasi waa sababta PCR waxaa loo isticmaalaa in cudurka si loo ogaado wareer ku filan yar yar, aqoonsiga of taas oo ah wax aan macquul aheyn waxa aan.

Waa in la ogaadaa in RNA ma noqon kartaa template ah PCR in waqtiga dhabta ah. Sidaa darteed, si ay u ogaan RNA viral ka hor cod ah in DNA xiran jab u dhigma waxaa ka mid ah. reaction silsilad polymerase wuxuu kuu ogol yahay in ay soo saaraan DNA iyo RNA pathogene cabiraada. Macluumaadka sidaas helay waxaa loo isticmaalaa isha waxtarka daawaynta loo isticmaalo, iyo qiimaynta saadaal caafimaad.

Intaa waxaa dheer,-waqtiga dhabta ah PCR uma baahna falalka dheeraad ah marka la eego decryption ee DNA ee diirada ama amplicons, kaas oo kuugu adeego sidii caqabad ku noqota cudurka saxda ah iyo mid horseeda in natiijada been ah. Habka Tani waxay suurtogal ahayn in la isticmaalo tallaabo electrophoresis ah, taas oo horseedaysa a alaabta khatarta-reduction cadaanyo silsilad polymerase ah. Sidoo kale, ay sabab u tahay hoos u dhac ah ee tirada manipulations la wakiilka, yaraynaysaa wakhtiga sahanka, waxa fududeyneysa habsocodka, yaraynaysaa suurtagalnimada qaladaad. PCR sidoo kale kuu ogolaanayaa inaad sheeg tirada hore ee nuqulo ka mid ah DNA ee pathogene ee tijaabada daawayn si ay u aqoonsadaan muddo of exacerbations iyo in ay qaadaan tallaabooyin ku habboon in lagu daaweeyo bukaanka.

Maanta, waxaa jira tiro badan oo ah hababka cod ah u qardarin in waqtiga dhabta ah. Farsamooyinka kala duwan ee inta badan la isticmaalo codsanayey amplicon xiran probes fluorogenic, xoojinta shucaaca kuwaas oo kala duwan taas oo ku xidhan raasamaal ee amplicon iyo diiwaan in waqtiga dhabta ah iyadoo loo adeegsanayo qalab gaar ah oo loo yaqaan thermocycler la module ah indhaha. Waxay bixisaa heer sare ah dareenka of falanqaynta. At soo saarka PCR isla waxaa lagu ogaan karaa, oo ay la socdaan tiro badan oo ah by-products, waqti marka la isticmaalayo waxaa electrophoresis mararka qaarkood aan la ogaaday.

By PCR isticmaalayo shaybaarka gacanta togan iyo kuwa taban ee waqtiga dhabta ah. Sayidka, shaybaarka togan iyo kuwa taban ee daraasad falanqeeyay baaritaanka gaar ah, tijaabada gacanta ku mudan dhexdeeda bandhigay in muunad kasta oo kale oo ag maraaba wuu dhammaan tallaabooyinka falanqaynta.

Maanta, waxaa jira tiro badan oo ah qalab loogu talagalay fulinta reaction silsilad polymerase ah. Kuwaas waxaa ka mid Bio-Rad, Applied Biosystems, Cepheid iyo Roche. Mid kasta oo iyaga ka mid ah waxay leedahay faa'iidada iyo khasaaraha

Waa in la ogaadaa in PCR ah - hab of diagnosis loo adeegsaday oo baaxad weyn sayniska, cilmi-baarista iyo xarumaha ogaanshaha adduunka oo dhan, maxaa yeelay waxa ay siinaysaa fursad ay ku fuliyaan fasiraadda degdeg ah natiijada.